Dear NIH-Korean Scientists, 

We will have the special NIH-KSA seminar on October 23rd. Lunch will be provided by ROKIT Healthcare, Inc.

We hope to see you there!

스페셜 NIH-KSA세미나가 10월 23일에 있을 예정입니다.

ROKIT Healthcare, Inc이 점심 제공합니다. 여러분의 많은 참여 부탁드립니다.

Time (강연시간): / October 23rd (Wed), 12:30-1:30 PM (45 min seminar, 15 min Q&A) 

장소: Campus, Building 10, FAES Room 10

SPEAKER INFORMATION

Name(성함): Joshua Jaeyun Kim, PhD (김재윤)

Position and Department: Sr. Director of Bio R&D, ROKIT Healthcare, Inc.

Seminar Title (강연제목):

Whole Human Extracellular Matrix (HumaTein) from primary Human Cell Culture as a Quality Controlled&Scalable Organ Specific Regenerative Biomaterial

Purpose

1)    To introduce ROKIT Healthcare, and its regeneration platforms. 

2)    To meet potential HumaTein customers.

3)    To find research opportunities.

Please email below for any questions and set up a separate meeting:

- Question: Joshua Jaeyun Kim (joshua@rokit.co.kr)  

- Separate meeting with speaker: Youngmi Ji (youngmi.ji@nih.gov) 

Abstract: 

Extracellular matrix (ECM), consisting of more than 300 proteins and associated cytokines, functions as a smart material; it provides not only a structural support, but also three-dimensional cues that exert profound effects on cell proliferation and phenotype maintenance. Most ECM proteins currently available for medical purposes a production process involving a series of chemical treatments and purifications. ECM thus produced is no longer , merely functioning as a space filler without promoting tissue regeneration. On the other hand, whole ECM currently in is derived from human or animal tissue. Although believed to be , this type of ECM especially from is not only banned for clinical use but also not specific to target tissue.

We developed a novel mode of ECM (HumaTein) preparation in which unmodified and undamaged whole human ECM was extracted from primary human as neonatal dermal fibroblasts (NDFBs) and mesenchymal stem cells growing in vitro. The -based culture was designed to simulate the native environment and found to have significant effects on cell phenotype and function. This whole human ECM contains cell-secreted natural proteins that ECM production and promotes tissue regeneration as well as structural reorganization. Compared to an alginate containing purified bovine collagen, the same type of reinforced with HumaTein showed a significantly increased ability to support endothelial proliferation and vascular network-like structure formation in a 3D printed construct. To further demonstrate the stem cell culturing potential of HumaTein, we cultured induced pluripotent stem cells (iPSCs) on a HumaTein-coated substrate. Gene expression analysis revealed that HumaTein enhanced the maintenance of IPS characteristic genes such as OCT4, SOX2, and E-cadherin. Moreover, HumaTein was able to replace Matrigel in colon culture to create Matrigel free implantable colon . Large animal test (porcine) indeed confirmed the regenerative potential of HumaTein, complete dermal regeneration and re- within 4 weeks. Furthermore, HumaTein delivery near using to 20 human subjects, 87% of volunteer reported more than 45% of wrinkle reduction. Taken together, our findings suggest HumaTein as a potent multi-purpose regenerative material that also capable to replace Matrigel system to create -free stem cell culture system.